Figure 5.

Recycling of the WT and triple mutant FSHR after agonist-provoked internalization (time zero), in the presence and absence of concanamycin A (lysosomal inhibitor), MG132 (proteasome inhibitor) or both. Total recycled FSH/FSHR complex increased upon exposure to MG132 but not concanamycin A (A,B). Meanwhile, the fraction of TCA soluble (degraded) ¹²⁵I-FSH in media from cells expressing the WT (C) or mutant FSHR (D) decreased in the presence of either MG132, concanamycin A or both (see Results). Different letters among bars indicate statistical differences (<0.05) within each time (0, 2, or 4 h).