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. 2016 Apr 12;6(4):336–343. doi: 10.1016/j.apsb.2016.03.010

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© 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — In vitro transfection efficiency and targeting ability studies of FA-SPE-PEG. Transfection efficiency in (A) KB/F cells and (B) A549 cells by FA-SPE-PEG/pGL3 at various weight ratios (mean±SD, n=3). (C) Buffering capacity study of FA-SPE-PEG/pGL3 in KB/F cells (mean±SD, n=3). Bafilomycin A1 (200 nmol/L) diluted in DMSO were put into wells. After incubation for 10 min, transfection solution was added into wells for 4 h. Then the cells were incubated in growth medium for 24 h. (D) Targeting capacity of FA-SPE-PEG/pGL3 in KB/F cells and KB cells (mean±SD, n=3). (E) GFP expression after KB/F cells were treated with FA-SPE-PEG/GFP and PEI 25K/GFP complexes. Composite images were made by overlapping the images of the individual channels.