Figure 2.

A, Morphological and phenotypic characteristics of rat bone marrow-derived mesenchymal stem cells (BM-MSCs). Stem cells exhibited large, flattened and fibroblast-like morphology (×40). B, Agarose gel electrophoresis of products of reverse transcriptase polymerase chain reaction (RT-PCR) revealing the presence of specific mesenchymal marker (CD73) compared with absence of specific hematopoietic marker (CD45) of rat BM-MSCs. C, BM-MSCs cultivated in osteogenic medium and stained with alizarin red (×100) and D, in adipogenic medium and were stained with Oil Red O at day 21 after induction (×100)