Figure 1.

Sixty minutes MCAO (middle cerebral artery occlusion). Schematic representation of the four 60 min MCAO experimental groups. The infusion of MB (methylene blue) (1 mg/kg) or Vehicle was given over 25–30 min beginning at 30 min and immediately after reperfusion at 60 min. NBO or Air was administered for 150 min after the baseline scans before and after reperfusion. MB (1 mg/kg) or Vehicle with 45–60 min NBO or Air was administered on Day 2, 7, and 14. Functional assessment was also performed on day 2, 7, 14, and 28.