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. 2016 Jul 20;6:29376. doi: 10.1038/srep29376

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Copyright © 2016, Macmillan Publishers Limited

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(A) recSIPC or BSA was incubated with chitin and then eluted using Milli-Q water, 0.5 M NaCl and a denaturation buffer (2% SDS + 50 mM DTT), sequentially. recSIPC was detected in the denaturation fraction but was absent in the NaCl fraction. (B) Chitins incubated with recSIPC solution were immunostained using a recSIPC antibody. The results strongly indicated recSIPC attachment to chitins. Chitins incubated directly with the recSIPC antibody and secondary antibody served as a control. These experiments were repeated three times and one group of images were presented.