Figure 4. mfd gene expression was assessed by measuring the ß-glucoronidase activity of the Bc407 [pHT304G-pmfd’gus] strain grown in LB medium at 37 °C with or without NO.

Samples were taken every hour from one hour before to one hour after the entry of the bacteria in the stationary growth phase. Specific activity is expressed in units of β-glucuronidase per milligram of protein (Miller units). The results reported are mean values of three independent experiments.