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. 2016 Jul 20;6:29589. doi: 10.1038/srep29589

Figure 1. tBHQ activates eNOS and Akt in HUVEC.

Figure 1

(A) HUVEC were treated with 50 μM tBHQ for the indicated times. The levels of Akt and eNOS phosphorylations in total cell lysates were analyzed by western blot. The blot is a representative of three blots obtained from separated experiments. Data presented are means ± SEM from 3 independent experiments. *P < 0.05 vs. control group (point 0). (B) HUVEC were treated with varying concentrations of tBHQ for 12 hours. The levels of Akt and eNOS phosphorylations in total cell lysates were analyzed by western blot. The blot is a representative of three blots obtained from separated experiments. Data presented are means ± SEM from 3 independent experiments. *P < 0.05 vs. control group (point 0). (C) eNOS activity was assessed by citrulline assay in (A). (D) eNOS activity was assessed by citrulline assay in (B). (E) HUVEC were pretreated with tBHQ (50 μM) for 30 minutes followed by AngII (1 μM) for 12 hours. Total cell lysates were analyzed by western blot for the indicated proteins. Data presented are means ± SEM from 3 independent experiments. *P < 0.05 vs. vehicle alone. #P < 0.05 vs. tBHQ alone. (F) HUVEC were treated with tBHQ (50 μM) for 12 hours. Total cell lysates were analyzed by western blot for the indicated proteins. The blot is a representative of three blots obtained from separated experiments.