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. 2016 Jul 20;6:29589. doi: 10.1038/srep29589

Figure 6. Deficiency of Akt abrogates tBHQ-induced improvement of endothelial dysfunction in AngII-infused mice.

Figure 6

WT and Akt−/− mice at the age of 8–12 weeks old were fed with normal diet containing tBHQ a ratio of 1% (w/w) for 2 weeks days prior to AngII infusion for another 14 days. Aortas from mice were cut into rings and were mounted in organ chamber to detect vessel bioactivity. The relaxation was induced acetylcholine (Ach) or SNP. (A) Endothelium-dependent relaxation of the aortic rings in response to Ach from WT mice. (B) Endothelium-independent relaxation of the aortic rings in response to SNP from WT mice. (C) Ach-induced endothelium-dependent relaxation in Akt−/− mice. (D) SNP-induced endothelium-independent relaxation in Akt−/− mice. Each data point represents relaxation expressed as a percentage of the value obtained for phenylephrine-preconstricted aorta. All data were expressed as mean ± SEM. One aortic ring was isolated from each mouse. 10–15 mice in each group. *P < 0.05 vs. Control WT or Akt−/− group. #P < 0.05 vs. AngII in WT mice.