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. 2016 Jul 20;6:29952. doi: 10.1038/srep29952

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Immunofluorescence analysis of PBS (left-hand panels) and Bleo (right-hand panels) mouse lungs at day 14 after instillation. Representative images with Gpx3 (red), Col-I (green), and DAPI (blue) are shown from bronchial (A) and alveolar regions (B) together with an isotype control for Gpx3 staining (C). Scale bar: 100 μm. Arrows in the higher magnification inserts indicate colocalization of Gpx3 with ECM structures: Subendothelial and subepithelial basement membrane (A) and interstitial ECM (B, only right-hand panel).