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. 2016 Jul 20;6:29952. doi: 10.1038/srep29952

Figure 8. Expression of glutathione peroxidase 3 (GPX3), is downregulated by TGF-β1 and TNF-α, but upregulated by the oxidative stress inducer menadione in primary human lung fibroblasts (phLF).

Figure 8

(A) Left panel: GPX3 transcript levels in phLF after treatment with 10 ng/ml TNF-α for 24 and 48 hours. Interleukin-6 (IL6) transcript is used as a positive control for TNF-α activity. Right panel: GPX3 protein levels in cell culture supernatant in response to TNF-α, as measured by ELISA. (B) Left panel: GPX3 transcript levels in phLF after treatment with 2 ng/ml TGF-β1 for 24 and 48 hours. Plasminogen activator inhibitor 1 (PAI1) transcript is used as a positive control for TGF-β1 activity. Right panel: GPX3 protein levels in cell culture supernatant in response to TGF-β1, as measured by ELISA. (C) Left panel: GPX3 and NQO1 transcript levels in differentiated phBEC after treatment with 10 μM menadione for 24 and 48 hours. Right panel: GPX3 protein levels in cell culture supernatant in response to menadione, as measured by ELISA. The presented data are based on four independent experiments (phLF isolated from four different donors) and given as mean ± SD. Statistical analysis was performed using paired t-test. *p < 0.1; **p < 0.01; ***p < 0.001.