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. 2016 Jul 20;6:29933. doi: 10.1038/srep29933

Figure 4. Myocardial regeneration and post-implantation in vivo reorganization of hiPSC-ECTs.

Figure 4

(a–c) Left ventricular (LV) histology 4 weeks after implantation of hiPSC-ECTs (including 5% MCs). (a) Masson’s trichrome staining (left). Red dotted line indicates engrafted area. cTnT and HNA (human cell marker) double immunostaining (right). Arrows indicate engrafted myocardium. White dotted lines indicate LV border. LV, left ventricle; HNA, human nucleic antigen. (b) Higher magnification of cTnT and HNA double immunostaining showing sarcomeric structure. (c) vWF (endothelial cell marker) and HNA double immunostaining. (i) Lower magnification image. White arrows indicate promoted capillary formation around grafted tissue. Orange arrows indicate penetrating vasculature. White dotted line indicates vasculature in the center of grafted tissue. (ii) and (iii) Higher magnification images. (ii) Prominent host-derived (HNA−) vascular formation around regenerated myocardium. (iii) chimeric vasculature composed of both host (HNA−; orange arrow) and graft (HNA+; white arrows) vascular cells. vWF, von Willebrand factor. Scale bars: 500 μm in (a), 200 μm in (c) (i), 50 μm in (c) (ii), 20 μm in (c) (iii), and 10 μm in (b).