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. 2016 Jul 20;6:30047. doi: 10.1038/srep30047

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Copyright © 2016, Macmillan Publishers Limited

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(A) The phenotypes of WT-3608, HD-29 and HOX-28 grown on PDA amended with 5 mM H₂O₂ (HP), 1 M mannitol (MAN) and 2% ethanol (ETN) for 14 days along with untreated control. (B) The colony diameter (mm) of all strains in the presence of 5mM H₂O₂ (HP), 1 M mannitol (MAN) and 2% ethanol (ETN) along with untreated control. The data were measured at 14 dpi. (C) The spore germination percentage after abiotic stress treatments. All strains were sensitive to 5 mM H₂O₂ (HP), 1 M mannitol (MAN) and 2% ethanol (ETN). (D) Transcription and relative expression of the HIM-SKN7 gene in response to 5 mM H₂O₂ (HP), 1M mannitol (MAN) and 2% ethanol (ETN) along with control. The WT-3608 were grown in PDB for 4 d and used for RT-PCR and qRT-PCR. The actin gene was used as an internal control. (E) The relative expression of heat shock transcription factors, HIM_00008 and HIM_00329, in WT-3608, knockout HD-29 and over-expressed HOX-28.