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. 2016 Jul 20;6:30047. doi: 10.1038/srep30047

Figure 5. Induction of apoptosis in H. minnesotensis.

Figure 5

(A) 10 days old germlings from WT-3608, HD-29 and HOX-28 were treated with farnesol (FOH) for 4 h at 25 °C, and the samples were double stained with Hoechst 33342/PI dyes. Fluorescence microscopy showed dose dependent apoptosis and necrosis of hyphae at 15 μM and 50 μM FOH. Bar = 50 μm. (B) The conidia survival assay on PDA treated with 50 μM FOH. (C) The conidia germination percentage of all strains exposed to FOH. HD-29 was more sensitive than WT-3608 and HOX-28 to 50 μM FOH.