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. 2016 Jul 20;6:29948. doi: 10.1038/srep29948

Figure 1. Molecular interaction between LeEILs and TERF1 promoter.

Figure 1

(a) Schematic diagram of TERF1 promoter, showing the position of PERE-like elements. TATA, TATA-box; ATG, initiation codon. (b) Binding of LeEILs to the PERE-like-element-containing fragment from TERF1 promoter in yeast. TERF1p, PERE-like-element-containing fragment. Transformants could grow on the selective medium (Growth, lacking histidine but containing 30 mM 3-AT) were subjected to filter-lifted β-galactosidase activity (β-galactosidase Activity). (c) In vitro interaction between LeEIL4 and the PERE-like elements in TERF1 promoter in gel-shift assay. GST-LeEIL4 indicates GST-tagged LeEIL4 protein, GST is control. Number 1, 2 and 3 indicate probes containing the PERE-like element at position -920–920, -857–851 and -787–781, respectively, and the corresponding unlabeled probes (Unlabeled probe) were used as competitive probes. Free probe indicates unbound probes. (d) Binding of LeEIL3/4 to TERF1 promoter in tobacco leaves. Left panel shows the structures of reporter and effector vectors, the effector vectors are driven by 35S promoter (CaMV35S). NOS, NOS terminator. The relative GUS activity values are the average of three independent triplicates. The GUS activity of non-effector control was set as 100. Error bars indicate ±SD. Asterisks indicate significant differences from the control (P < 0.005, Student’s t test).