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. 2016 Jul 20;6:29948. doi: 10.1038/srep29948

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(a) NBT staining for superoxide in tobacco leaves. (b) DAB staining for H₂O₂ in tobacco leaves. Control indicates untreated seedlings. Ethylene indicate ethylene-treated seedling. Approximately 15 seedlings were used for each line, and the representative pictures of three replicates are shown.