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. 2016 Jan 14;99(6):797–810. doi: 10.1189/jlb.2HI0415-146RR

Figure 1. Activation of Gal4-RARA by natural retinoids.

Figure 1.

(A) 293T cells were transfected, as indicated; treated with liarozole, DEAB, or ATRA; and analyzed for GFP expression after 72 h. (B) Model of stepwise ATRA synthesis and effect of inhibitors. (C and D) Activation of UAS-GFP reporter by MSCV-Gal4-RARA-IC in Kit+ progenitor cells. (E and F) Activation of UAS-GFP reporter by MSCV-Gal4-RARA-IC in BMMφ. In each case, cells were prepared, transduced with MSCV-Gal4-RARA-IC, and then treated as indicated for 72 h before analysis of GFP. Error bars represent sd of triplicate experiments.