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. 2016 Jan 14;99(6):797–810. doi: 10.1189/jlb.2HI0415-146RR

Figure 7. Stra6 in BMMφ.

Figure 7.

(A) Semiquantitative RT-PCR of Stra6 and Oaz1 in RNA extracted from spleen, liver, kidney, total bone marrow (BM) cells, and Kit+ progenitor cells and in BMMφ. PCR was performed with 50 ng total RNA starting material/well and 34 cycles. Expected fragment size Stra6, 379 bp; Oaz1, 332 bp. (B) Schema of BMMφ transduction and evaluation. (C and D) After transduction with MSCV-STRA6-IRES-Cerulean, Cerulean-negative BMMφ were transduced with Gal4-RARA-IC and treated with or without mouse serum for 48 h before evaluation. (E and F) Cerulean-positive BMMφ were transduced with MSCV-Gal4-RARA-IC and treated with or without mouse serum for 48 h before evaluation. Representative data of 2 independent experiments.