Table 2.
aSpot no. | bAccession number | cProtein name | dZT/ZC | dJT/JC |
---|---|---|---|---|
Up-regulated proteins | ||||
Spot5 | gi|460372520 | Oxygen-evolving enhancer protein 1 | 2.21* ± 0.44 | 10.57* ± 0.52 |
Spot10 | gi|100380 | Ribulose bisphosphate carboxylase activase | 3.25* ± 0.44 | 5.57* ± 0.43 |
Spot41 | gi|350535160 | Wound-inducible carboxypeptidase precursor | 3.62* ± 0.30 | 5.27* ± 0.22 |
Spot42 | gi|350535160 | Wound-inducible carboxypeptidase precursor | 2.72* ± 0.19 | 6.05* ± 0.30 |
Spot54 | gi|350536267 | Subtilisin-like protease precursor | 4.17* ± 0.29 | 14.46* ± 0.40 |
Spot55 | gi|460381101 | 28 kDa ribonucleoprotein | 2.17* ± 0.70 | 2.05* ± 0.25 |
Spot60 | gi|460399143 | Glycine dehydrogenase (decarboxylating) | 4.02* ± 0.25 | 5.51* ± 0.19 |
Spot61 | gi|460399143 | Glycine dehydrogenase (decarboxylating) | 6.81* ± 0.38 | 5.09* ± 0.21 |
Spot63 | gi|170458 | Threonine deaminase, partial | 5.90* ± 0.09 | 7.29* ± 0.29 |
Spot64 | gi|170458 | Threonine deaminase, partial | 5.28* ± 0.62 | 2.96* ± 0.28 |
Spot65 | gi|170458 | Threonine deaminase, partial | 4.35* ± 0.03 | 7.62* ± 0.14 |
Spot68 | gi|460389504 | Protein disulfide-isomerase-like | 3.51* ± 0.28 | 3.39* ± 0.22 |
Spot69 | gi|460395973 | Heat shock protein 83 | 19.73* ± 0.24 | 9.53* ± 0.23 |
Spot77 | gi|590715109 | Transducin family protein / WD-40 repeat family protein isoform 1 | 2.87* ± 0.03 | 12.76* ± 0.11 |
Spot81 | gi|350536897 | Cytosolic ascorbate peroxidase 1 | 6.03* ± 0.16 | 5.85* ± 0.07 |
Spot85 | gi|251895 | Polyphenol oxidase | 15.32* ± 0.10 | 10.49* ± 0.42 |
Spot86 | gi|460397526 | Heme-binding protein 2 | 4.29* ± 0.25 | 4.41* ± 0.18 |
Down-regulated proteins | ||||
Spot1 | gi|407970998 | Chlorophyll a-b binding protein 4 | 0.22* ± 0.13 | 0.18* ± 0.09 |
Spot3 | gi|460405507 | Chlorophyll a-b binding protein 8 | 0.46* ± 0.03 | 0.36* ± 0.13 |
Spot8 | gi|723739979 | Ribulose bisphosphate carboxylase/oxygenase activase | 0.07* ± 0.05 | 0.16* ± 0.01 |
Spot11 | gi|488453358 | Ribulose 1,5-bisphosphate carboxylase | 0.04* ± 0.01 | 0.01* ± 0,01 |
Spot12 | gi|488453358 | Ribulose 1,5-bisphosphate carboxylase | 0.06* ± 0.01 | 0.02* ± 0.01 |
Spot13 | gi|488453358 | Ribulose 1,5-bisphosphate carboxylase | 0.05* ± 0.01 | 0.05* ± 0.01 |
Spot14 | gi|488453358 | Ribulose 1,5-bisphosphate carboxylase | 0.09* ± 0.01 | 0.04* ± 0.01 |
Spot15 | gi|1293000 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | 0.10* ± 0.05 | 0.06* ± 0.01 |
Spot17 | gi|21069067 | Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit | 0.02* ± 0.01 | 0.09* ± 0.08 |
Spot20 | gi|92087012 | Ribulose bisphosphate carboxylase large chain OS | 0.09* ± 0.01 | 0.01* ± 0.01 |
Spot21 | gi|92087012 | Ribulose bisphosphate carboxylase large chain OS | 0.08* ± 0.01 | 0.01* ± 0.01 |
Spot22 | gi|460375527 | Ferredoxin--NADP reductase, leaf-type isozyme | 0.42* ± 0.10 | 0.14* ± 0.02 |
Spot27 | gi|460372959 | Peptidyl-prolyl cis-trans isomerase CYP38 | 0.26* ± 0.05 | 0.15* ± 0.04 |
Spot28 | gi|469517896 | Glyceraldehyde-3-phosphate dehydrogenase | 0.46* ± 0.03 | 0.44* ± 0.13 |
Spot29 | gi|460414390 | ATP synthase delta chain | 0.38* ± 0.05 | 0.11* ± 0.03 |
Spot37 | gi|460373820 | ATP synthase gamma chain | 0.09* ± 0.01 | 0.05* ± 0.01 |
Spot39 | gi|350535679 | Cytosolic NADP-malic enzyme | 0.48* ± 0.07 | 0.49* ± 0.20 |
Spot43 | gi|350534564 | 26S protease regulatory subunit 6A homolog | 0.20* ± 0.07 | 0.30* ± 0.04 |
Spot46 | gi|460415494 | Eukaryotic initiation factor 4A-2 | 0.35* ± 0.02 | 0.37* ± 0.02 |
Spot47 | gi|460399092 | Eukaryotic initiation factor 4A-2 | 0.49* ± 0.22 | 0.22* ± 0.05 |
Spot57 | gi|460404838 | Adenosylhomocysteinase | 0.21* ± 0.06 | 0.45* ± 0.19 |
Spot74 | gi|1168191.1 | 14-3-3 protein 4 OS | 0.10* ± 0.04 | 0.11* ± 0.05 |
Spot75 | gi|3041662 | 14-3-3 protein 3 | 0.21* ± 0.04 | 0.08* ± 0.02 |
Spot76 | gi|460405902 | Plasma membrane-associated cation-binding protein 1 | 0.20* ± 0.04 | 0.34* ± 0.04 |
Opposite expression proteins | ||||
Spot31 | gi|460397188 | Thiamine thiazole synthase | 2.25* ± 0.07 | 0.23* ± 0.17 |
Spot33 | gi|723747143 | Aconitate hydratase | 0.26* ± 0.02 | 5.77* ± 0.85 |
Spot45 | gi|460400419 | Elongation factor G | 0.47* ± 0.01 | 3.29* ± 0.39 |
Spot48 | gi|460391817 | Elongation factor TuB | 2.45* ± 0.23 | 0.34* ± 0.03 |
Spot51 | gi|460391351 | Cell division cycle protein 48 homolog | 0.38* ± 0.04 | 5.90* ± 0.81 |
Spot62 | gi|460370413 | Glycine dehydrogenase | 3.33* ± 0.16 | 0.07* ± 0.02 |
Spot66 | gi|460398434 | Cysteine synthase | 2.11* ± 0.22 | 0.33* ± 0.04 |
Spot83 | gi|460373807 | Putative lactoylglutathione lyase | 0.44* ± 0.21 | 2.05* ± 0.24 |
aNumbering corresponds to the 2-DE in Fig. 3
bAccession number from the NCBI nr database
cNames of the proteins obtained via the MASCOT software from the NCBI nr database
dProtein abundance ratio of Treatment/Control tomato cultivars, with each value representing the mean value ± SD of three biological replicates
∗Indicates significant (more than 2.0-foldor less than 0.50-fold) difference between control and treatment tomato cultivars