Figure 4.

Activation of HOXB4 resulted in a modest increase in the proportion of immature CD235a⁺/CD71⁺ erythroid cells. HOXB4 was activated with 4OHT from days 10 to 24 (A). Production of erythroid cells was monitored by expression of CD235a and CD71 by flow cytometry at day 24 (B–D). Data were generated from four independent experiments with error bars representing the SEM. ∗, p = .05. Abbreviations: 4OHT, tamoxifen; BMP4, bone morphogenetic protein 4; C, cell; CFU, colony-forming unit; EPO, erythropoietin; FGF a, fibroblast growth factor-α; FLT3L, FMS-like tyrosine kinase receptor 3 ligand; GSK, glycogen synthase kinase; IBMX, isobutylmethylxanthine; IGF1, IGF2, insulin-like growth factor 1, 2; IL, interleukin; Q, quartile; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.