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. 2016 Jul 20;3:16050. doi: 10.1038/mtm.2016.50

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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FACS-based purification plots of human induced pluripotent stem cells (hiPSCs) and OCT4_SRE activity. (a) The ^High35% Stb/eGFP-double positive fraction post-2x FACS SORT was collected. (b) Indirect OCT4_SRE-mediated Stb expression assessment was performed after OCT4 activation by reprogramming to hiPSC by ICC staining for RFP where blue = DAPI, RFP = Stb expression driven by OCT4_SRE, eGFP = EF1α-eGFP. (Bar = 200 μm). eGFP, enhanced green fluorescent protein; EF1α, elongation factor 1α; FACS, fluorescence-activated cell sorting; hiPSC, human induced pluripotent stem cell; OCT4, octamer-binding transcription factor 4; OCT4_SRE, OCT4-short response element.