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. 2015 Dec 9;10(3):238–246. doi: 10.1080/19336950.2015.1124311

Figure 4.

Figure 4.

Effect of PIP3 on subcellular localization of β2e and MARCKS. (A) Confocal images of cells expressing LDR and CF-PTEN with Btk-PH, β2e-GFP, or MARCKS-GFP before and after the addition of 1 μM rapamycin for 2 mins. (B) Time courses of the effects of PIP3 depletion on cytosolic translocation of Btk-PH, β2e-GFP, and MARCKS-GFP. For analysis, n=4. (C) Current inactivation of CaV2.2 channels upon PIP3 depletion. Currents were measured during a 500-ms test pulse to +10 mV. For quantification, n=5. (D) Confocal images of cells expressing Btk-GFP with β2e-mCh or MARCKS-mCh in the absence or presence of dominant-negative p85 (Δp85). Bar, 5 μm.