Table 1.
Identified Protein | Gene | Average number of unique peptides (total spectra) | Average % amino acid sequence coverage | |
---|---|---|---|---|
WT | Fgf14−/− | |||
Voltage-gated Na+ channel α subunit Nav1.2 | Scn2a | 58 (158) | 0 | 27 |
Voltage-gated Na+ channel α subunit Nav1.1 | Scn1a | 35 (73) | 0 | 24 |
Voltage-gated Na+ channel α subunit Nav1.6 | Scn8a | 23 (45) | 0 | 17 |
FGF14 | Fgf14 | 10 (26) | 0 | 51 |
Calmodulin | Calm | 9 (33) | 2 (3) | 55 |
Voltage-gated Na+ channel β2 subunit | Scn2b | 9 (21) | 0 | 35 |
Voltage-gated Na+ channel α subunit Nav1.4 | Scn4a | 2 (12) | 0 | 3 |
Synaptotagmin-1 | Syt1 | 2 (3) | 0 | 16 |
Synaptotagmin-2 | Syt2 | 15 (32) | 2 (2) | 57 |
Voltage-gated Na+ channel β1 subunit | Scn1b | 8 (17) | 0 | 28 |
Ankyrin R | Ank1 | 4 (4) | 0 | 3 |
Ankyrin G | Ank3 | 9 (10) | 0 | 6 |
Inositol 1,4,5-trisphosphate receptor type 1 | Itpr1 | 4 (4) | 0 | 2 |
Voltage-dependent anion-selective channel protein 1 | Vdac1 | 2 (2) | 0 | 7 |
Vesicle-associated membrane protein-associated protein A | Vapa | 2 (4) | 0 | 13 |
Voltage-gated Na+ channel β4 subunit | Scn4b | 2 (3) | 0 | 7 |
Excitatory amino acid transporter 1 | Slc1a3 | 5 (7) | 2 (2) | 14 |
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 | Atp2a2 | 2 (3) | 0 | 3 |
Casein kinase II subunit β | Csnk2b | 1 (1) | 0 | 5 |
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit β−1 | Gnb1 | 1 (1) | 0 | 4 |
Solute carrier family 12 member 5 | Slc12a5 | 1 (1) | 0 | 1 |
Protein-glutamine gamma-glutamyltransferase 2 | Tgm2 | 1 (1) | 0 | 3 |
Neurofascin | Nfasc | 1 (1) | 0 | 1 |
14-3-3 protein theta | Ywhaq | 1 (1) | 0 | 14 |
Synaptosomal-associated protein 25 | Snap25 | 1 (1) | 0 | 9 |
Note. The average number of exclusive unique peptides and total spectra as well as the average percent amino acid sequence coverage obtained for each protein (from a total of 4 runs in each condition) are presented. Rows in bold indicate voltage-gated Na+ (Nav) channel α subunits or previously identified Nav channel interacting proteins.