Table 2.
Proteins identified in native WT and Fgf14−/− cerebellar Nav channel complexes.
| Identified Protein | Gene | Number of unique peptides (total spectra) | % amino acid sequence coverage from WT (Fgf14−/−) IPs | |
|---|---|---|---|---|
| WT | Fgf14−/− | |||
| Voltage-gated Na+ channel α subunit Nav1.2 | Scn2a | 18 (28) | 26 (44) | 12 (16) |
| Calmodulin* | Calm | 8 (27) | 8 (29) | 31 (31) |
| Cofilin-1 | Cfl1 | 6 (11) | 7 (12) | 46 (51) |
| Microtubule-associated protein 6 | Map6 | 6 (8) | 11 (13) | 8 (14) |
| Reticulocalbin-2 | Rcn2 | 4 (4) | 5 (7) | 20 (25) |
| Purkinje cell protein 2 | Pcp2 | 3 (5) | 3 (4) | 32 (32) |
| Voltage-gated Na+ channel β2 subunit | Scn2b | 3 (3) | 3 (3) | 16 (16) |
| Polymerase delta-interacting protein 3 | Poldip3 | 3 (3) | 9 (11) | 11 (35) |
| Myosin light polypeptide 6 | Myl6 | 3 (3) | 5 (5) | 24 (38) |
| Voltage-gated Na+ channel α subunit Nav1.1 | Scn1a | 2 (3) | 4 (4) | 2.9 (5) |
| Tubulin α−1B chain | Tuba1b | 2 (3) | 3 (5) | 6 (10) |
| ATP synthase-coupling factor 6, mitochondrial | Atp5j | 2 (3) | 2 (3) | 35 (35) |
| ADP/ATP translocase 1 | Slc25a4 | 2 (3) | 2 (3) | 8 (8) |
| Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 | Chchd3 | 2 (2) | 0 | 10 (0) |
| Translation initiation factor IF-2, mitochondrial | Mtif2 | 1 (1) | 2 (3) | 2 (4) |
| 78 kDa glucose-regulated protein | Hspa5 | 1 (1) | 5 (6) | 2 (9) |
| 2-oxoisovalerate dehydrogenase subunit α, mitochondrial | Bckdha | 0 | 9 (12) | 0 (26) |
| ATP synthase subunit d, mitochondrial | Atp5h | 0 | 3 (3) | 0 (28) |
| RNA-binding protein FUS | Fus | 0 | 2 (2) | 0 (5) |
Notes. The number of exclusive unique peptides and total spectra as well as the percent amino acid sequence coverage obtained for each protein are presented. Rows in bold indicate voltage-gated Na+ (Nav) channel α subunits or previously identified Nav channel interacting proteins.
*
There were also calmodulin peptides identified in the mIgG-IPs from WT (n = 3 peptides) and Fgf14−/− (n = 2 peptides) cerebellar lysates.