Figure 1.
AE2 activity is regulated by SPL (A and B) HeLa cells were transfected with indicated plasmids. Cells in Solution A were exposed to HCO3−-buffered solutions. Cl−/HCO3− exchanger (CBE) activity was assessed by slope of pHi in the absence of Cl− at the beginning of time course (30˜45 sec) and height to reach the maximum pHi point from the minimum point. The traces were the mean ± SEM of number of experiments under each condition. (*P < 0.01). (C) The Co-IP of AE2 with SPL. AE2 was tagged with HA and SPL was tagged with GFP. Anti-HA, anti-GFP and anti-SPL antibodies were used for the Co-IP and detection of proteins. β-Actin was used as a control. (D) Analysis of surface expression of AE2. The bar graphs show the mean ± SEM. The (-) represented cell lysates.