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. 2016 Feb 22;10(4):258–260. doi: 10.1080/19336950.2016.1155900

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — The activity of the TRPM3 channel reconstituted in planar lipid bilayers. The model and recordings of TRPM3 in the bilayers: Top traces: Basal TRPM3 activity, regularly obtained and characterized by its complete inactivation within ˜10 min. Middle traces: TRPM3 activity induced by 5 μM pregnenolone sulfate (PS) in the presence of 5 μM phosphatidylinositol-4, 5-bisphosphate (PIP₂). Lower traces: Heat-induced TRPM3 exhibits low open probability in the presence of PIP₂. All recording were obtained at 100 mV.