Figure 1.

Sequence alignments of the S6 domains of NavAb (Arcobacter butzleri), NavAe1 (Alkalilimnicola ehrlichei), NavMs (Magnetococcus marinus), NavRh (Rickettsiales sp HIMB114) and NaChBac (Bacillus haldurans), as well as from domains I to IV of human Nav1.2, are shown as determined using ClustalW2. F1764 and Y1771 in Nav1.2 DIV (bright blue) are implicated in the binding of local anesthetic drugs.²⁵ The corresponding aromatic residues in each sequence alignment are indicated with pale blue boxes and the NavAb residues, T206 and V213, are indicated in the black outlined box. It should be noted that 3 of the 4 domains in Nav1.2 have an aromatic residue at the position equivalent to 1771 in DIV. All other aromatic residues are indicated by a lilac box.