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. 2016 Jul 20;11(7):e0158212. doi: 10.1371/journal.pone.0158212

Fig 6. Recognition of Pfs48/45 in fixed P. falciparum parasites in indirect immunofluorescence assays.

Fig 6

The representative results for fixed parasites incubated with pooled pre-immune sera, pooled sera from CFA adjuvant alone immunized mice, a representative anti-Pfs48/45 serum, a representative ELISA and WB positive anti-Pvs48/45 serum (CFA group), and a representative ELISA and WB positive anti-Pvs48/45 (Montanide ISA-51 group) at a dilution of 1:100. BF, bright field; DAPI, the nuclei stained by NucBlue reagent; FITC, antibody reactivity to the parasite visualized with FITC-conjugated anti-mouse IgG antibody, and Merge of DAPI and FITC images. All images were visualized and captured at 1000X magnification. Scale bar (white line), 5μm.