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. 2016 Jul 20;11(7):e0159517. doi: 10.1371/journal.pone.0159517

Fig 1. Experimental Design.

Fig 1

NSG mice were used to construct BLT humanized mice. The peripheral blood (PB) of BLT mice were monitored longitudinally for human immune reconstitution. BLT mice were infected with HIV-1JR-CSF (30,000 TCIU) intravenously (IV, day 0). Beginning at 3 weeks post-HIV infection, BLT mice (n = 6) were administered EFdA (10mg/kg) once daily for three weeks via oral gavage. Control (untreated) mice (n = 6) were administered PBS orally. PB and CVL were collected weekly from EFdA-treated and untreated mice for the analysis of HIV-RNA and CD4+ T cell levels. EFdA-treated and untreated mice were necropsied at 6 weeks post-infection and tissues harvested for analysis of HIV-RNA, HIV-DNA and CD4+ T cell levels.