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. 2016 Jul 20;11(7):e0158724. doi: 10.1371/journal.pone.0158724

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© 2016 Bromley et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A. Induction of CYP51A in parental (A1163) and deletion mutant (Δ29.9) isolates 4 h after addition of 1 mg/L itraconazole (Δ29.9+I and A1160+I) or DMSO solvent control (Δ29.9 and A1160). B. Induction of CYP51B in parental (A1160) and deletion mutant (Δ29.9) isolates 4h after addition of 1 mg/L itraconazole (Δ29.9+I and A1160+I) or DMSO solvent control (Δ29.9 and A1160). C. Ergosterol levels of parental (A1160), knockout (Δ29.9) CYP51A (CYP51A) knockout, CYP51B knockout (CYP51B) and reconstituted knockout (Δ29.9::29.9) strains 4 h after addition of a range of itraconazole concentrations (levels given as mg/l in panel C). Results represent averages of three biological replicates with three technical replicates for each. Error bars shown represent standard deviation.