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. 2016 Jul 20;11(7):e0159426. doi: 10.1371/journal.pone.0159426

Fig 1. Generation of Tg mice expressing human CD89: CD14 gene knock-in strategy.

Fig 1

The targeting vector contains 2.6kb of DNA homologous to the 5’ and 3’ sequence of the mouse CD14 gene (with blue boxes representing coding region and green boxes non-coding region), 1kb of 2A-CD89 cDNA (yellow box and blue arrow) and a frt-neo-frt cassette. Homologous recombination between the targeting vector and the endogenous CD14 gene in the mouse ES cell results in the insert of the whole 2A and CD89 region. The CD89 transgenic mice were intercrossed with FLP mice to excise the neo cassette.