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. 2016 Jul 20;11(7):e0159426. doi: 10.1371/journal.pone.0159426

Fig 7. IgA clearance induced by CD89.

Fig 7

(A) Enhanced efficiency of a 60-min serum clearance challenge by 10 μg human mIgA1 in C57BL/6-Tg mice. A total of 10 μg of human mIgA1 was injected into the tail vein of C57BL/6-Tg or WT mice (n = 5 per group). At the indicated times post-injection, sera were obtained and IgA levels were determined. IgA cleared more quickly in Tg mice(*). (B) IgA clearance by CD89 on Kupffer cells of CD89 transgenic mice. A total of 10 μg of FITC-labeled human mIgA was injected into the tail vein of C57BL/6-Tg mice (n = 5 per group). Mice were sacrificed after 10 min or 1 h, livers were stained with rabbit anti–human CD89 mAb and Cy3-goat anti-rabbit IgG, as indicated. (C) IgA clearance by CD89 on Kupffer cells from CD89 transgenic mice analyzed in vitro. Cy3-labeled human mIgA (1 μg/mL) was incubated with Kupffer cells from CD89 transgenic mice for 1h at 37°C.