Figure 6. Ceapin-A7 sensitizes cells to ER stress.
(A–B) U2-OS cells were treated with increasing concentrations of ER stressor (Tg) in the absence (black) or presence of six micromolar Ceapin analogs - Ceapin-A5 (inactive, blue) or Ceapin-A7 (purple). (A) After seventy-two hours reducing potential of living cells was assayed to determine cell viability. Data plotted are the means of four independent experiments performed in triplicate, error bars represent the standard error of the mean. (B) EC50 values calculated for ER stressor in the absence or presence of Ceapin analogs showing mean and 95% confidence limits. (C). U2-OS cells were treated with increasing concentrations of ER stressor (Tg) in the absence (black) or presence of 6 μM Ceapin-A7 (purple). To analyze cell death, cells were stained with Annexin V and 7AAD and analyzed by flow cytometry. Data plotted are the means from three independent experiments performed in duplicate; error bars represent standard deviation.