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. 2016 May 16;37(6):731–740. doi: 10.1038/aps.2015.154

Figure 5.

Figure 5

Effects of 20C on the Nrf2/ARE/HO-1 signaling pathway. (A) Western blotting analysis of the levels of the Nrf2 protein in the cytoplasm and nucleus of PC12 cells exposed to rotenone in the presence or absence of various concentrations of 20C. (B) Quantitative analysis of the density of Nrf2 in the cytoplasm and nucleus. (C) Confocal microscopy images of immunofluorescence with an anti-Nrf2 antibody showed the nuclear translocation of Nrf2 in the 20C-treated PC12 cells. The scale bar represents 10 μm. (D, E) Western blotting analysis of the levels of the HO-1 and NQO1 proteins in PC12 cells exposed to rotenone in the presence or absence of 20C. (F, G) RT-PCR analysis of the mRNA levels of HO-1 and NQO1 in PC12 cells exposed to rotenone in the presence or absence of 20C. #P<0.05, ##P<0.01 vs the model group (n=3).