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Enrichment of phage-display nanobodies by panning. Phage-display nanobodies were selected against biotin-VEGFR2 D3 protein and E coli TG1 was infected with bound phage. The number of phages eluted after each round of panning was counted based on the number of colonies (CFU/mL) formed after reinfection of the host bacteria with the eluted phage particles.
