Figure 6. The adjuvant effect of Alum-TLR7 requires functional TLR7.

Nine mice per group were immunized at day 1 and 28 with MenC-CRM₁₉₇/Aluminium Hydroxide or MenC-CRM₁₉₇/Alum-TLR7. 21 days after the first (A) and 14 days after the second (B) immunization, sera were collected and total IgG specific for the MenC polysaccharide were measured by ELISA. Values for each mouse in all groups and the geometric mean (bar) with 95% CI are reported in the graph. Mann Whitney test was applied: *P < 0.01. (C) 14 days after the second immunization, splenocytes were stimulated in vitro with CRM₁₉₇ and the percentages of Ag-specific cytokine-secreting CD4⁺ T cells were analyzed by intracellular staining. Bars show the average of 9 mice per group of CD4⁺ T cells producing IL-17 with/without IL-2 and TNFα (Th17); IL-2 and/or TNFα (Th0); INF-γ with/without IL-2 and TNFα (Th1); IL-4+IL-13 with/without IL-2 and TNFα (Th2).