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. 2016 Jul 21;6:30070. doi: 10.1038/srep30070

Figure 6. Agomir-17-5p also accelerated the free-running rhythm of mice.

Figure 6

(A,B) Representative actograms of C57BL/6J mice that underwent i.c.v. injection of the control (agomir-NC) or miR-17-5p agomir (agomir-17-5p) at CT10. The activity onsets are depicted by coloured oblique lines, and the injection times are indicated by red asterisks. (C, D) Display of free-running periods of an individual mouse before and after the i.c.v. injection of control (C) or miR-17-5p agomir (D). (E) Mean values of delta free-running periods (delta tau), which were −16.27 ± 3.01 min in the mice injected with agomir-17-5p and −6.93 ± 1.63 min in the control animals. *P = 0.0150 vs. scrambled, n = 9 per group. (F) Western blotting indicates the effects of miR-17-5p on clock gene expression in N2a cells steadily transfected with control vector or pcDNA3.1-miR-17 (left) and control sponge or miR-17-5p sponge (right). Note that the overexpression or subtraction of miR-17-5p increased the CRY1 expression in N2a cells. (G) Western blotting indicates the overexpression and interference of Clock augmented CRY1 protein. (H) Immunochemical stains of CRY1 in the SCN of mice at CT12 underwent i.c.v. injections of miR-17-5p antagomirs or agomirs.