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. 2016 Jul 21;6:30127. doi: 10.1038/srep30127

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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t-MCAO rats were intravenously injected with FK506-liposomes (100 μg/kg FK506 equivalent) or PBS immediately after reperfusion. Seven days after reperfusion, 10-μm frozen brain sections were prepared and immunostained for NeuN (green) and counterstained nuclei with DAPI (blue). The fluorescence images of the striatum (a) and the cortex (b) were obtained by confocal laser scanning microscopy. Isc and non-isc indicate ischemic and non-ischemic areas, respectively. Scale bar, 50 μm. Quantitative data from confocal images were obtained by counting NeuN-positive cells in the sections (c). Data are presented as the mean ± S.D. (n = 5). Significance of differences: *P < 0.05, ***P < 0.001 vs. PBS.