Figure 2.

Cytokine production. (a) Interleukin (IL)‐12, (b) interferon (IFN)‐γ and (c) IL‐10 in stimulated peripheral blood mononuclear cells (PBMCs) of cured visceral leishmaniasis (VL) patients (n = 8) and endemic controls (n = 6) in response to recombinant Leishmania donovani S‐adenosyl‐L‐homocysteine hydrolase (rLdAdoHcy) antigens; each data point represents one individual. The x‐axis represents the group of individuals (cured and endemic) and the y‐axis corresponds to the values of respective cytokine concentrations in pg/ml. The mean concentration of cytokine for each group is indicated by the horizontal bars. Cytokine production was tested in triplicate in two independent experiments and the results were comparable. The lower detection limits for various cytokines were as follows: 5·1 pg/ml for IFN‐γ, 30·8 pg/ml for IL‐12 and 6·9 pg/ml for IL‐10.