Figure 6.

Analysis of mRNA expression profile of recombinant Leishmania donovani S‐adenosyl‐L‐homocysteine hydrolase (rLdAdoHcy) + bacillus Calmette–Guérin‐vaccinated hamsters by quantitative real time reverse transcription–polymerase chain reaction (qRT–PCR). Splenic inducible nitric oxide synthase (iNOS) (a) and cytokines, interleukin (IL)−12 (b), interferon (IFN)‐γ (c), tumour necrosis factor (TNF)‐α (d), IL‐4 (e), IL‐10 (f) and transforming growth factor (TGF)‐β (g), mRNA expression profile was assessed by qRT–PCR in all the experimental groups of hamsters on days 45 and 90 post‐challenge (p.c. Data are presented as means ± standard deviation (s.d.) and are representative of two independent experiments with similar results. Significance values indicate the difference between the vaccinated and unvaccinated infected control groups (*P < 0·05; **P < 0·01; ***P < 0·001).