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. 2016 Jun 18;6(10):1629–1640. doi: 10.7150/thno.15253

Figure 1.

Figure 1

PD-1 immunoPET/CT in naive mice. Frequency of PD-1+ and PD-1- cells in CD45+ leukocytes and the absolute cell count of PD-1+ and PD-1- cells among CD4 and CD8 T cells, CD19 B cells, and other leukocytes in spleen (A) and lymph nodes (B); n = 3 each. (C) Representative density dot plots of splenic CD4/CD8 T cells from C57BL/6 and PD-1-/- mice i.v. injected with α-PD-1-AF680 (RMP1-14), ex vivo counterstained with α-PD-1-PE (29F.1A12). (D) Representative coronal (C) and transverse (T) immunoPET/CT sections from WT, antigen-blocked, and PD-1-/- mice. White ticks in the C-sections indicate the position of the T-section. Scanning duration was ca. 45-50 min. (E) Uptake of 64Cu-NOTA-PD-1 mAb as measured by microPET in various organs of WT, antigen-blocked, and PD-1-/- mice 24 h p.i. of 1.5 µg tracer (1.13 ± 0.31 MBq). Antigen-blocked mice were pretreated with unlabeled α-PD-1 (1 mg, i.v.) 24 h before; n = 6 (WT), 5 (Block), and 3 (PD-1-/-). (F) Ex vivo biodistribution of radiotracer uptake 48 h p.i.; n = 14 (WT), 11 (Block), and 3 (KO). Abbreviations: cLN - cervical lymph node, aLN - axillary lymph node, iLN - inguinal lymph node, mLN - mesenteric lymph node, Sp - spleen, Thym - thymus, Panc - pancreas, Stom - stomach, Intest - intestines, Blad - bladder, BAT - brown adipose tissue.