Table 2.
Standard curve and quality control analyses.
| Analyte | dA | dTG |
|---|---|---|
| LOD (ng injected) a | ndf | 0.0003125 |
| LLOQ (ng) b | ndf | 0.000625 |
|
analytical range (ng injected) |
0.0975 ng to 50 ng | 0.000625 ng–10 ng |
| average curvec | Y = −6218126 ×2 + 26200326x + 5014429 | y = 263992 × + 148.9 |
| weighting | 1/x2 | 1/x |
| fit | quadratic | linear |
| Intra-day High QCd | 3.5% | 4.2% |
| Intra-day Mid QC | 7.8% | 7.7% |
| Intra-day Low QC | 3.3% | 11.9% |
| Inter-day High QCe | 7.8% | 8.2% |
| Inter-day Med QC | 5.1% | 8.5% |
| Inter-day Low QC | 17.0% | 15.9% |
a
Limit of detection (10 × baseline).
b
Lower Limit of Quantification (2 × LOD).
c
Calibration curves adjusted R2: dA = 0.991; dTG = 0.996.
d
Intra-day, n = 10.
e
Inter-day, n = 5.
f
nd, not determined: dA standard concentrations were 100-fold higher than for dTG and sensitivity for dA was not relevant.