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. 2016 Jul 20;17:16. doi: 10.1186/s12867-016-0069-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Response of promoter activity to increasing dose of SREBP1a or C/EBPα or −β transcription factors in HepG2 or MAC-T cells. Reporter genes (1 µg) with the long or short promoter segment (PL, PS) were transfected into HepG2 or MAC-T cells, together with increasing amounts (abscissa) of vectors expressing the respective transcription factor. RLUs (ordinate, untreated control set as 1) were determined 48 h after transfection, as described above. The total amount of transfected DNA was kept constant by eventually filling up with empty vector. Values are means calculated from two independent determinations, each assayed in triplicate. S.E.M. error bars of the mean values were always smaller than the size of the symbols. All, but the values for 100 ng of transfected SREBP were significantly different from the controls