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. 2016 Jul 20;9:147. doi: 10.1186/s13068-016-0560-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — SDS-PAGE analysis of the purified recombinant β-glucosidase Bgl3A with and without deglycosylation treatment. a Coomassie Blue staining. Lanes M, the standard protein molecular weight markers; 1 the Bgl3A produced in P. pastoris; 2 the Bgl3A produced in P. pastoris and treated with PNGase F; 3 the Bgl3A produced in P. pastoris and treated with PNGase F followed by α-mannosidase; 4 the α-mannosidase; 5 the purified Bgl3A after sequential deglycosylation by PNGase F and α-mannosidase; 6 the Bgl3A produced in E. coli. b Periodic Acid-Schiff staining