Table 2.

Kinetic parameters of β-glucosidase Bgl3A and its two mutants

Substrate	Enzyme	Specific activity (U/mg)	K i (mM)	K m (mM)	V max (U/mg)	k cat (/s)	k cat/K m (/s/mM)
pNPG	Bgl3A	905	14.0	0.18	1309	1664	9096
	M1	1019	17.0	0.31	1408	1791	5778
	M2	843	12.3	0.36	1679	2136	4664
Cellobiose	Bgl3A	265.5	ND	10.4	618.4	786.0	75.8
	M1	209.4	ND	8.4	531.6	676.0	80.5
	M2	197.6	ND	9.3	733.0	932.6	100.3

ND not determined