Table 2.
Xylose and glucose initial uptake kinetics of fungal MFS transporters
| Transporter | Xylose initial uptake kinetics | Glucose initial uptake kinetics | References | ||
|---|---|---|---|---|---|
| V max (nmol min−1 mg DW−1) | K m (mM) | V max (nmol min−1 mg DW−1) | K m (mM) | ||
| AnXltA | 1.08 ± 0.05 | 0.09 ± 0.03 | 1.11 ± 0.15 | 0.07 ± 0.01 | This study |
| AnXltB | 0.10 ± 0.00 | 15.0 ± 4.50 | ND | ND | This study |
| AnXltC | 0.14 ± 0.01 | 4.71 ± 1.04 | 1.18 ± 0.15 | 0.11 ± 0.02 | This study |
| TrStr1 | 0.04 ± 0.01 | 5.70 ± 0.19 | 0.14 ± 0.04 | 0.01 ± 0.00 | This study |
| TrStr2 | 0.12 ± 0.02 | 6.18 ± 0.81 | 0.69 ± 0.19 | 0.05 ± 0.01 | This study |
| TrStr3 | 0.46 ± 0.04 | 2.19 ± 0.29 | 1.31 ± 0.33 | 0.06 ± 0.01 | This study |
| AnMstA | NCa | 0.3 ± 0.1 | NC | 0.03 ± 0.01 | [38] |
| GXS1 | 0.01 ± 0.00 | 0.08 ± 0.02 | – | – | [17] |
| XUT3 | 0.01 ± 0.00 | 4.09 ± 1.08 | – | – | [17] |
| Xyp29 | 0.61 ± 0.05 | 175.74 ± 21.36 | ND | ND | [26] |
| An25 | 0.69 ± 0.04 | 55.96 ± 9.37 | ND | ND | [26] |
| SUT1 | 132.0 ± 1.0 | 145.0 ± 1.0 | 45.0 ± 1.0 | 1.5 ± 0.1 | [23] |
| SUT2+ | 41.0 ± 1.0 | 49.0 ± 1.0 | 3.3 ± 0.1 28.0 ± 4.0 |
1.1 ± 0.1 55 ± 11.0 |
[23] |
| SUT3+ | 87.0 ± 2.0 | 103.0 ± 3.0 | 3.7 ± 0.1 22.0 ± 0.1 |
0.8 ± 0.1 31.0 ± 0.1 |
[23] |
| Ag11C3 | ND | ND | ND | ND | This study |
Ag11C3; laboratory-evolved xylose-utilizing strain, transformed with the empty vector p426HXT7-6His
Italics: originating from filamentous fungi
+ Two glucose transport components were reported
− Not determined
ND not detectable
NC not comparable
aValues were reported in an incompatible unit (nmol min−1 mg protein−1)