Fig. 6.

Effects of LINC00261 on gastric cancer cell migration and invasion in vitro and in vivo. BGC823 cells were transfected with pcDNA3.1-LINC00261, and MGC803 cells were transfected with si-LINC00261. a Wound healing assays were used to investigate the migratory ability of gastric cancer cells. Experiments were performed in triplicate. Bars: SD; **P < 0.01. b Transwell assays were used to investigate changes in the migratory and invasive abilities of gastric cancer cells. Experiments were performed in triplicate. Bars: SD; *P < 0.05 and **P < 0.01. c, d Stable BGC823 cells transfected with pcDNA3.1-LINC00261 and an empty vector were separately injected into the tail veins of athymic mice. The lungs were harvested from the mice in each experimental group, and tumor nodules visible on lung surfaces were counted. The assay was independently conducted twice. Bars: SD; *P < 0.05 and **P < 0.01. e Visualization of the entire lung and hematoxylin and eosin (HE)-stained lung sections