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. 2015 Feb 26;127(15):4691–4694. doi: 10.1002/ange.201410670

Table 2.

PAL variants with the highest specific d‐formation activity.[a]

Variant Specific d‐PAL activity [U mg−1] Relative activity compared to WT
WT 3.23 –
H359Y 11.35 3.52
H359K 10.76 3.34
S456P 9.83 3.05
A88Q 9.10 2.82
S263A 7.95 2.47

[a] Reaction conditions: 5 mm 1 a, 5 m NH4OH, 25 μg mL−1 purified PAL, pH 9.6, 37 °C, 220 rpm, 4 h.