Table 3.
Effects of AF and honey on antioxidant defense system and MDA contents in various tissues of rats
| Tissue | Parameters | A (Control) | B (AF-treated) | C(AF + Honey-treated) |
|---|---|---|---|---|
| Mean ± SD | Mean ± SD | Mean ± SD | ||
| Erythrocyte | GSH mg/dl | 39.5 ± 0.84 | 38,8 ± 1,2 | 43,8 ± 1,5 |
| MDA nmol/ml | 0,39 ± 0,08 | 0,69 ± 0,2a | 0,49 ± 0,1ab | |
| CAT U/ml | 461.1 ± 40.4 | 112.6 ± 12.1a | 401.4 ± 147.1b | |
| GST U/ml | 0,62 ± 0,16 | 0,75 ± 0,39 | 0,35 ± 0,21 | |
| GR U/ml | 0.37 ± 0.12 | 0.7 ± 0.34a | 0.85 ± 0.29a | |
| SOD U/ml | 428,6 ± 10,5 | 443,5 ± 3,5 | 436,5 ± 13,5 | |
| Brain | GSH mg/g | 28.4 ± 4.4 | 32.5 ± 5.4 | 33.7 ± 3.8 |
| MDA nmol/g | 34.1 ± 2.4 | 38.1 ± 3.4 | 38.3 ± 4.7 | |
| CAT U/g | 54.9 ± 13.3 | 52.3 ± 21.5 | 55.6 ± 12.2 | |
| GST U/g | 2.6 ± 0.3 | 2.6 ± 0,3 | 2.8 ± 0,5 | |
| GR U/g | 0.94 ± 0.2 | 0.83 ± 0.14 | 0.73 ± 0.07 | |
| SOD U/g | 1511.7 ± 115.2 | 1605.1 ± 55.3 | 1631.6 ± 55.3 | |
| Liver | GSH mg/g | 27,5 ± 2,1 | 21,8 ± 0,5a | 24,6 ± 1,5 |
| MDA nmol/g | 35,4 ± 3,04 | 73,7 ± 13,8a | 49,1 ± 8,1ab | |
| CAT U/g | 79,7.4 ± 13.2 | 30.8 ± 13.7a | 66.3 ± 17.6b | |
| GST U/g | 8,8 ± 1,2 | 8,7 ± 2,7 | 8,5 ± 1,6 | |
| GR U/ml | 1.34 ± 0.1 | 0.85 ± 0.11a | 0.80 ± 0.19a | |
| SOD U/g | 1879,7 ± 36,8 | 1451,9 ± 275a | 1582,5 ± 90a | |
| Kidney | GSH mg/g | 46.5 ± 0.6 | 45.7 ± 1.9 | 45.7 ± 1.8 |
| MDA nmol/g | 6.3 ± 2.5 | 72.9 ± 43.8a | 11.9 ± 3.1ab | |
| CAT U/g | 24.1 ± 11.9 | 24.8 ± 14.7 | 20.7 ± 2.8 | |
| GST U/g | 6.5 ± 1.1 | 5.3 ± 0,3 | 4.6 ± 0,4 | |
| GR U/g | 0.37 ± 0.1 | 0.26 ± 0.1 | 0.3 ± 0.1 | |
| SOD U/g | 1504.7 ± 161.8 | 1485.6 ± 303.6 | 1468.3 ± 250.7 | |
| Heart | GSH mg/g | 36.4 ± 0.9 | 39.6 ± 2.3 | 41.1 ± 1.7 |
| MDA nmol/g | 7.12 ± 1.2 | 21.8 ± 1.2a | 8.3 ± 3.1b | |
| CAT U/g | 22.8 ± 1.5 | 16.7 ± 4.1 | 16.7 ± 6.3 | |
| GST U/g | 2.5 ± 0.19 | 2.8 ± 0,2 | 2.17 ± 0,3 | |
| GR U/g | 1.2 ± 0.16 | 1.15 ± 0.06 | 1.15 ± 0.06 | |
| SOD U/g | 1349.3 ± 69.4 | 1471.3 ± 78.5 | 1467.9 ± 8.2 | |
| Lungs | GSH mg/g | 39.9 ± 4.8 | 27.8 ± 3.5a | 39.4 ± 3.2 |
| MDA nmol/g | 11.8 ± 2.8 | 73.4 ± 11.8a | 42.3 ± 6.1ab | |
| CAT U/g | 43.5 ± 14.2 | 37.5 ± 17.6 | 37.5 ± 22.3 | |
| GST U/g | 6.2 ± 1.3 | 5.7 ± 1,2 | 3.3 ± 0,5 | |
| GR U/g | 1.3 ± 0.14 | 1.5 ± 0.08 | 1.2 ± 0.2 | |
| SOD U/g | 1879.8 ± 36.8 | 1643.6 ± 112.1a | 1582.6 ± 89.9a |
Each value represents the Mean ± SD. a: significantly different from control, b: significantly different from B group rats at p < 0.05 (One way ANOVA)