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. 2016 Jul 20;16:506. doi: 10.1186/s12885-016-2526-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The Prognostic significance of EpCAM ^mRNA+ CTC and Treg/CD4⁺ ratio in HCC patients. a. Kaplan–Meier analysis of HCC patients receiving curative resection treatment according to relative preoperative EpCAM expression <2.22 or ≥2.22 (2^−ΔΔC _q algorithm transformation). b. Kaplan–Meier analysis of patients with HCC undergoing curative resection according to preoperative Treg/CD4+ <5.07 % or ≥5.07 %. c. Prognostic significance of combination of EpCAM ^mRNA+ CTC and Treg/CD4⁺ load with respect to time to recurrence in patients with high EpCAM ^mRNA+ CTC (≥2.22) and high Treg/CD4⁺(≥5.07 %), high EpCAM ^mRNA+ CTC(<2.22) and low Treg/CD4⁺(<5.07 %), low/no EpCAM ^mRNA+ CTC (<2.22) and high Treg/CD4⁺(≥5.07 %), and low/no EpCAM ^mRNA+ CTC (<2.22) and low Treg/CD4⁺(<5.07 %)