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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Mol Microbiol. 2016 Feb 10;100(3):425–441. doi: 10.1111/mmi.13326

Fig. 6. Role of Ssa1 in hyphae formation and regulation of the CEK and PKC pathways.

Fig. 6

A. Cells were grown in YNB + 2% Glu for 3 h at 37°C or 42°C. Cells were examined by microscopy at 20X magnification. Arrows refer to examples of hyphae at 42°C. Bar, 10 microns.

B. Cells were grown to mid-log phase and incubated in YNB + GlcNac media pre-warmed to 37°C for 3 h at the indicated temperatures. Total cellular protein (20 µg) from cell lysates was examined by immunoblot analysis with α-phospho p42/44 MAPK, α-Cek1 and α-Act1 antibodies (as a control for protein loading).

C Wild type cells and the indicated mutants were incubated in YPD or YPD with 10 µM GDA at 30°C for 6 h. Cells were examined by microscopy at 20X magnification. Bar, 10 microns.